Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://rgd.mcw.edu/tools/strains/strains_view.cgi?id=626168255
Proper Citation: RRID:RRRC_00974
Description: Rattus norvegicus with name SD-Tg(Thy1-APPDI)BWevn from RGD.
Species: Rattus norvegicus
Notes: This novel transgenic rat produces low levels of human familial cerebral amyloid angiopath (CAA) Dutch/Iowa E22Q/D23N mutant amyloid beta protein in brain and recapitulates many of the pathologic aspects of human small-vessel CAA. A pcDNA3 vector containing 2.1 kb of human APP (isoform 770) cDNA was used to introduce the mutations Swedish K670N/M671L, Dutch E693Q, and Iowa D694N using the QuikChange kit. The APP770-SwDI cDNA was amplified by PCR using primers containing the NheI five linker and SacII three linker. The PCR product of the APP770-SwDI was subcloned between exons II and IV of a Thy1.2 expression cassette using NheI and SacII restriction sites. The completed construct was microinjected into pronuclei of Sprague-Dawley single-cell embryos. Founder rats were identified by Southern blot analysis of tail DNA. Transgenic offspring were determined by PCR analysis of tail DNA. All subsequent analyses were performed with hemizygous transgenic rats. Rat Resource and Research Center
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for SD-Tg(Thy1-APPDI)BWevn .
No alerts have been found for SD-Tg(Thy1-APPDI)BWevn .
Source: Integrated Animals
Source Database: Rat Resource and Research Center (RRRC)
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
