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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=626168247
Proper Citation: RRID:RGD_626168247
Description: Rattus norvegicus with name WI-Tg(Avp-CHRM3*-mCherry)Tuch from RGD.
Species: Rattus norvegicus
Notes: A chimeric AVP-hM3Dq-mCherry BAC clone transgene construct was purified for microinjections. hM3Dq is a modified form of the human M3 muscarinic receptor (hM3; CHRM3*). It can be activated by the inert clozapine metabolite clozapine-N-oxide (CNO), engaging the Gq signaling pathway.The hM3Dq-mCherry sequence from the hM3Dq-mCherry cassette (Plasmid #44361, Addgene, Cambridge, MA, USA) was used for the transgene. Next, SV40 poly A sequence was framed to the hM3Dq-mCherry sequence. Finally, this hM3Dq-mCherry-SV40 poly A cassette was introduced into the rat Avp gene in place of the genomic start codon. Thus, hM3Dq-mCherry should be specifically expressed under the Avp promoter in the transgenic rat brain.
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Source: Rat Genome Database (RGD) (RRID:SCR_006444)
Description: Database for genetic, genomic, phenotype, and disease data generated from rat research. Centralized database that collects, manages, and distributes data generated from rat genetic and genomic research and makes these data available to scientific community. Curation of mapped positions for quantitative trait loci, known mutations and other phenotypic data is provided. Facilitates investigators research efforts by providing tools to search, mine, and analyze this data. Strain reports include description of strain origin, disease, phenotype, genetics, immunology, behavior with links to related genes, QTLs, sub-strains, and strain sources.
URL: http://rgd.mcw.edu
Source Database: Rat Genome Database (RGD)
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
