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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=598154612
Proper Citation: RRID:RGD_598154612
Description: Rattus norvegicus with name SD-Tg(TOR1ADYT1)Tot from RGD.
Species: Rattus norvegicus
Notes: The 16.25 kbps full length human DYT1 allele of TOR1A gene including upstream and downstream flanking regions were inserted into a pUC19 vector. Following linearization and purification the transgenes were used to generate transgenic rats by microinjection of the DNA construct into the pronucleus of rat zygotes. Transgenic rats were kept on Sprague–Dawley background and bred as hemizygotes using standard procedures. Genotypes of offspring were determined by PCR and sequencing of DNA extracted from ear biopsies. Primer pair 5′-TAAAAATGTGTATCCGAGTGGAAAT (forward) and 5′-AAGGACTGAGTGTTGTTTCTTTTC (reverse) amplified a 230 bp segment within the coding sequence of exon 5 under the following conditions: 94 °C for 1 min, 60 °C for 1 min and 72 °C for 1 min, 40 cycles. Only lines that showed stable germline transmission of the transgenes were used for further analyses.
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Source: Rat Genome Database (RGD) (RRID:SCR_006444)
Description: Database for genetic, genomic, phenotype, and disease data generated from rat research. Centralized database that collects, manages, and distributes data generated from rat genetic and genomic research and makes these data available to scientific community. Curation of mapped positions for quantitative trait loci, known mutations and other phenotypic data is provided. Facilitates investigators research efforts by providing tools to search, mine, and analyze this data. Strain reports include description of strain origin, disease, phenotype, genetics, immunology, behavior with links to related genes, QTLs, sub-strains, and strain sources.
URL: http://rgd.mcw.edu
Source Database: Rat Genome Database (RGD)
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
