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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=35668859
Proper Citation: RRID:RGD_35668859
Description: Rattus norvegicus with name W-Gnrh1tm1(cre)Aeh from RGD.
Species: Rattus norvegicus
Notes: This model was generated using zinc finger nuclease (ZFN) method. Cre recombinase was expressed under the control of the endogenous Gnrh1 gene by inserting an internal ribosomal entry site (IRES)-Cre cassette 30bp downstream from the translational stop site of the Gnrh1 open reading frame. This was achieved by pronuclear co-injection of zinc finger nucleases (ATCCACAACATCCGAGTGtgacattGACGCTGAGATCCATGAC; cleavage site in lower case) along with a donor plasmid containing two 800bp homologous arms (HA) flanking IRES-Cre into a one-cell stage rat embryo. (1.) Allan Herbison at Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.; (2.) Vincent Prevot in INSERM, Lille, France.
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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