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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=127345126
Proper Citation: RRID:RGD_127345126
Description: Rattus norvegicus with name F344-Angptl8em2Kyo+/- from RGD.
Species: Rattus norvegicus
Notes: This line #2 Angptl8 heterozygous rats were generated using the CRISPR/Cas9 system containing two guide RNAs (gRNAs) targeting exons 2 and 3 in the rat Angptl8 gene. A mixture of transcribed Cas9 and gRNAs was microinjected into F344/Stm rat zygotes [National BioResource Project rat number: 0140) provided by the National BioResource Project for the Rat in Japan. Two lines of rats heterozygous for Angptl8 (lines #1 and #2). Male and female Het rats were intercrossed to obtain homozygous Angptl8 KO rats. Rats were genotyped by PCR with the following primers5'-GGGTGAGCAAAGCTGACCTA-3' (sense) and 5'-GAGTAAACCCACCAGGCTCA-3' (antisense) for line #2. A 980-bp deletion in the ANGPTL8 gene was identified in line # 2, resulting in a premature termination codon. National BioResource Project for the Rat in Japan
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
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