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URL: http://www.addgene.org/87359
Proper Citation: RRID:Addgene_87359
Insert Name: C321.∆A.opt strain
Organism: Synthetic
Bacterial Resistance: Gentamicin
Defining Citation: PMID:28545477
Vector Backbone Description: Vector Backbone:Whole genome; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin
Comments: C321.∆A.opt is a variant of C321.∆A (Addgene Item #48998) engineered to have a faster growth rate, as reported in Kuznetsov, Gleb, et al. "Optimizing complex phenotypes through model-guided multiplex genome engineering." bioRxiv (2016): 086595. This strain has a heat-inducible lambda prophage (42 C) and should be grown at or below 34 C to avoid induction. The original strain C321.∆A (Addgene Item #48998) was reported in Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013): 357-360.
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Source: Addgene