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Plasmid Name
C321.∆A.opt
RRID:Addgene_87359 RRID Copied  
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RRID:Addgene_87359
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Plasmid Information

URL: http://www.addgene.org/87359

Proper Citation: RRID:Addgene_87359

Insert Name: C321.∆A.opt strain

Organism: Synthetic

Bacterial Resistance: Gentamicin

Defining Citation: PMID:28545477

Vector Backbone Description: Vector Backbone:Whole genome; Vector Types:Synthetic Biology; Bacterial Resistance:Gentamicin

Comments: C321.∆A.opt is a variant of C321.∆A (Addgene Item #48998) engineered to have a faster growth rate, as reported in Kuznetsov, Gleb, et al. "Optimizing complex phenotypes through model-guided multiplex genome engineering." bioRxiv (2016): 086595. This strain has a heat-inducible lambda prophage (42 C) and should be grown at or below 34 C to avoid induction. The original strain C321.∆A (Addgene Item #48998) was reported in Lajoie, Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013): 357-360.

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Data and Source Information

Source: Addgene