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URL: http://www.addgene.org/60006
Proper Citation: RRID:Addgene_60006
Insert Name: Control (F+E) sgRNA
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25336740
Vector Backbone Description: Vector Backbone:pCESAx; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: U6 promoter based on Nishiyama, A. and Fujiwara, S. (2008). RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo. Dev. growth … 521–529. sgRNA F+E scaffold based on Chen, B., Gilbert, L. a, Cimini, B. a, Schnitzbauer, J., Zhang, W., Li, G.-W., Park, J., Blackburn, E. H., Weissman, J. S., Qi, L. S., et al. (2013). Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell 155, 1479–91.
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Source: Addgene
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