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URL: http://www.addgene.org/40834
Proper Citation: RRID:Addgene_40834
Insert Name: Four target sites perfectly complementary to let-7
Organism: Drosophila melanogaster
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20558712
Vector Backbone Description: Backbone Marker:Murhpy Lab, Carnegie Institution for Science; Backbone Size:7233; Vector Backbone:pAW; Vector Types:Insect Expression, miRNA reporter; Bacterial Resistance:Ampicillin
Comments: The let-7 complimentary sequence was inserted in the XbaI site in the 3'UTR of EGFP using the following oligos: s: CTA GAA CTA TAC AAC CTA CTA CCT CAT CTA GAA CTA TAC AAC CTA CTA CCT CAT as: CTA GAT GAG GTA GTA GGT TGT ATA GTT CTA GAT GAG GTA GTA GGT TGT ATA GTT This expression cassette was then sub-cloned into the Gateway vector pAW using GATEWAY cloning.
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No rating or validation information has been found for pAW-EGFP-4xlet-7 (pAW-AS27).
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Source: Addgene
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