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URL: http://www.addgene.org/38069
Proper Citation: RRID:Addgene_38069
Insert Name: MYEF2 myelin expression factor 2
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22681889
Vector Backbone Description: Backbone Size:5508; Vector Backbone:pFRT/TO/HIS/FLAG/HA-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Digest and NGS results suggest that the original Gateway entry vector is still present in the DNA stock from which the bacterial stocks are created. Because the entry vector does not contain a promoter for the insert or selection marker, it should not interfere with function. To attempt to remove the entry vector, recipient scientists could try to cure the plasmid from isolated colonies. Alternatively, they can linearize the entry vector with BmtI, HpaI or NheI digest, which will not cut the expression vector, and retransform into bacteria.
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Source: Addgene
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