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URL: http://www.addgene.org/30471
Proper Citation: RRID:Addgene_30471
Insert Name: CMVenh/gp91/eGFP/cHS4
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21544095
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLV; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Comments: From the paper (note that some of these features may not be in this plasmid): All vectors were based on the pRRL-cPPT-hPGK-mcs-WPRE vector, derived from the pRRL-cPPT-hPGKeGFP-WPRE construct. The gp91promoter is the natural myeloid-specific promoter. The CMVenh comes from the peGFP-N1(Clontech, Saint-Germain-en-Laye, France), the synthetic intron from the pMG5 and the EF1a intron from the pWPI vector http://www.addgene.org/12254 The myeloid-specific enhancer SP107 and SP146 were provided by Senlin Li (Department of Medicine, UTHSCSA, San Antonio, TX, USA). The cHS4 insulator corresponds to the chicken beta-globin 5’-DNase I-hypersensitive site and was provided by Patrick Salmon (Department of Neuroscience, Faculty of Medicine, University of Geneva, Switzerland).
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Source: Addgene
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