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Plasmid Name
pBCN27-Pmyo-2::GFP::unc-54_3'UTR
RRID:Addgene_26347 RRID Copied  
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RRID:Addgene_26347
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Plasmid Information

URL: http://www.addgene.org/26347

Proper Citation: RRID:Addgene_26347

Insert Name: Pmyo-2::GFP::unc-54_3'UTR

Organism: Caenorhabditis elegans

Bacterial Resistance: Ampicillin

Defining Citation: PMID:20729840

Vector Backbone Description: Backbone Size:10813; Vector Backbone:pBCN27-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin

Comments: The full sequence provided is theoretical sequence based on a three way gateway reaction. The order of the elements has been confirmed by PCR, but not fully sequence verified. Expression pattern in worms is as expected. Use this plasmid as a positive control for testing puromycin selection after single copy insertion by MosSCI at the ttTi5605 locus.

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Data and Source Information

Source: Addgene