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URL: http://www.addgene.org/26346
Proper Citation: RRID:Addgene_26346
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Vector Backbone Description: Backbone Size:10809; Vector Backbone:pCJF150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The Prpl-28::PuroR::let-858_3'UTR cassette was inserted into the backbone of pCFJ150 to create a puromycin-selectable MosSCI vector for single copy integration in worms at the ttTi5605. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
