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URL: http://www.addgene.org/22310
Proper Citation: RRID:Addgene_22310
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19745116
Vector Backbone Description: Backbone Size:5857; Vector Backbone:pYES2.1 modified; Vector Types:Yeast Expression, Integrating plasmid; Bacterial Resistance:Ampicillin
Comments: A MCS containing XhoI and EcoRI sites was cloned between the PvuII and XbaI sites of pYES2.1. The empty vector was created by replacing the hairpin of pIp-strongSC_GFP with XhoI and EagI sites To make this plasmid integrating, the 2micron and f1 origins were replaced (using NheI and SpeI sites with sequence from S. castellii sc633:288301-289016 (amplified from genomic DNA with 5'-AAAAGCTAGCGATCCCTTATCAAATATGGTAC and 5'-AAAAACTAGTGTAGAATCCAGAGAATAGAATC). Please note that Addgene's sequencing results differ from the full plasmid sequence from the depositing laboratory by a single nucleotide mismatch at bp# 3135 in the vector backbone. This mismatch is not known to affect plasmid function.
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Source: Addgene
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