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Plasmid Name
pHis-TEV-Nsp15-E266Q
RRID:Addgene_218803 RRID Copied  
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RRID:Addgene_218803
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Plasmid Information

URL: http://www.addgene.org/218803

Proper Citation: RRID:Addgene_218803

Insert Name: Nsp15-E266Q

Organism: Severe acute respiratory syndrome coronavirus 2

Bacterial Resistance: Ampicillin

Defining Citation: PMID:40411374

Vector Backbone Description: Backbone Marker:purchased from GenScript; Backbone Size:5644; Vector Backbone:pET-11b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: Protein sequence is MHHHHHHSSGVDLGTENLYFQSNAM + Nsp15-E266Q (amino acid residues 6453-6798 of YP_009724389, with E266Q mutation). Confers ampicillin-resistance. Backbone vector is pET-11b from GenScript. Mutation design by Nirupa Nagaratnam.

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Data and Source Information

Source: Addgene