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URL: http://www.addgene.org/216788
Proper Citation: RRID:Addgene_216788
Insert Name: CVB3
Organism: Coxsackievirus B3
Bacterial Resistance: Gentamicin
Defining Citation: PMID:39536108
Vector Backbone Description: Vector Backbone:pEN_TTmcs; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Comments: To maintain the CVB3 internal EcoRI site, this plasmid was created by in a two step procedure: i) PCR cloning of CVB3 from its internal EcoRI site (nucleotides 2764-2769) to the end (nucleotide 7399), digesting with EcoRI-MfeI, and ligating into pEN_TTmcs (Addgene #25755) digested with EcoRI; ii) PCR cloning of CVB3 from its start (nucleotide 1) to the internal EcoRI site, digesting with EcoRI, and ligating into the intermediate plasmid (i) digested with EcoRI.
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Source: Addgene
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