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URL: http://www.addgene.org/159446
Proper Citation: RRID:Addgene_159446
Insert Name: BsMDH BmHPS BmPHI (on Addgene plasmid # 129104)
Organism: Bacillus subtilis and Bacillus methanolicus (for plasmid gene insert)
Bacterial Resistance: Ampicillin and Kanamycin
Defining Citation: PMID:33404828
Vector Backbone Description: Backbone Marker:Eleftherios Terry Papoutsakis (Addgene plasmid # 129104); Vector Backbone:pETM6_Ptrc_BsMdh_BmHps_BmPhi; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This T-B18 E.coli strain harbors the pETM6_Ptrc_BsMdh_BmHps_BmPhi plasmid (Addgene plasmid # 129104), which enables the evolved E. coli strain to metabolize methanol in its central carbon metabolism. It was adaptively evolved from the parent strain, E. coli BW25113 ΔfrmA::kanR (obtained from the Keio collection) containing Addgene plasmid # 129104 such that it grows efficiently with just 2mM Thr and 250 mM MeOH. This strain has been functionally validated for growth by the depositing laboratory. Addgene has not confirmed the plasmid by QC sequence analysis.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
