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URL: http://www.addgene.org/15024
Proper Citation: RRID:Addgene_15024
Insert Name: Ngn3 promoter
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11973276
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision. To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library (Genetics Institute, Buffalo, NY). Three primers were used to fuse the Cre-ERTM-coding region to the Ngn3 ATG to generate a fragment that had part of the Ngn3 promoter and the 5' end of the recombinase. The primers used are p73 (5'-acacttgactccttgatcgctg-3'), p71 (5'-gttgcatcgaccggtaatgca-3') and p74 (5'-ttttcccaaccgcaggatgtccaatttactgaccgtacac-3'). This fragment was subsequently digested with ApaI and AgeI and ligated to the KpnI (blunt-ended)-ApaI (partial digestion)-digested pGD15, and a 2.4 kb AgeI-SacII (partial digestion, blunt-ended) fragment of pGD35. The insert from the final construct (pGD29) was released by NotI digestion. See Author's Map and article for more information.
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Source: Addgene (RRID:SCR_002037)
Description: Non-profit plasmid repository dedicated to helping scientists around the world share high-quality plasmids. Facilitates archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Repository contains over 65,000 plasmids, including special collections on CRISPR, fluorescent proteins, and ready-to-use viral preparations. There is no cost for scientists to deposit plasmids, which saves time and money associated with shipping plasmids themselves. All plasmids are fully sequenced for validation and sequencing data is openly available. We handle the appropriate Material Transfer Agreements (MTA) with institutions, facilitating open exchange and offering intellectual property and liability protection for depositing scientists. Furthermore, we curate free educational resources for the scientific community including a blog, eBooks, video protocols, and detailed molecular biology resources.
URL: http://www.addgene.org
The NIDDK Information Network (dkNET) serves the needs of basic and clinical investigators by providing seamless access to large pools of data and research resources relevant to the mission of The National Institute of Diabetes Digestive and Kidney Diseases (NIDDK). dkNET is hosted at University of California San Diego, and is supported by NIH NIDDK grant U24DK097771.
Email: info_at_ dknet.org
