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URL: http://www.addgene.org/90048
Proper Citation: RRID:Addgene_90048
Insert Name: LGhG
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19818797
Vector Backbone Description: Backbone Marker:PMID: 3012611 with lacO1 binding site of pHG165 disabled by mutation; Vector Backbone:pHG165a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: E. coli LacI DNA binding domain, E. coli GalR linker region, and GalR regulatory domain . The start codon of these genes is GTG, which is an alternative MET start codon in E. coli. The GalR insert contains an E230K mutation compared to wild type.
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Source: Addgene
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