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URL: http://www.addgene.org/89632
Proper Citation: RRID:Addgene_89632
Insert Name: xylE
Bacterial Resistance: Gentamicin
Defining Citation: PMID:17074913
Vector Backbone Description: Backbone Marker:PMID: 9380741; Backbone Size:8550; Vector Backbone:pPR23; Vector Types:Allelic change mutagenesis; Bacterial Resistance:Gentamicin
Comments: SacB is used for counter-selecton in Mycobacteria to force excision and loss of this plasmid to occur. This plasmid has been used as an allelic exchange vector. We have used SpeI site for cloning of an insert (flanking regions of a target gene to be deleted), so other groups can also use SpeI (NotI and XbaI can be used as well).
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Source: Addgene
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