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URL: http://www.addgene.org/83036
Proper Citation: RRID:Addgene_83036
Insert Name: none
Organism: Other
Bacterial Resistance: Tetracycline
Defining Citation: PMID:25853840
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Tetracycline
Comments: Precursor strain: ElectroMAX DH10B E. coli cells Landing pad cassette at at the aptI/gidB locus includes in this order: I-SceI restriction site - Landing Pad 1 (LP1) sequence - tetA gene - Landing Pad 2 (LP2) sequence - I-SceI restriction site. This strain was made based on the technology described in the Kulman and Cox 2010 Nucleic Acids Res. paper: Site-specific chromosomal integration of large synthetic constructs. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2847246/
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Source: Addgene
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