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URL: http://www.addgene.org/75239
Proper Citation: RRID:Addgene_75239
Insert Name: sgRNA against NFATc1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:27637333
Vector Backbone Description: Backbone Marker:Feng Zhang (Plasmid #48140); Backbone Size:9703; Vector Backbone:PX461-pSpCas9n(BB)2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Comments: gRNA contains at least 3 missmatches against human DNA (http://crispr.mit.edu). 2 additional nonmatching Guanidines added at 5' to enhnace specificy (Kim, D. et al. Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–43, 1 p following 243 (2015).) targets all isoforms. MUST BE COTRANSFECTED WITH PLASMID #75238
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No rating or validation information has been found for NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP).
No alerts have been found for NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP).
Source: Addgene
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