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URL: http://www.addgene.org/71510
Proper Citation: RRID:Addgene_71510
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:23328393
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL4.10; Vector Types:Mammalian Expression, humanSTARR-seq validation vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Note: The plasmid for enhancer-activity testing by luciferase assays has been used in the Arnold et al. 2013 publication. It should be replaced for all enhancer activity tests mammalian cells with the updated version reported by Muerdter et al. 2017. The improved version is Addgene plasmid # 99297. For enhancer-activity measurements in mammalian cells, please also consider the possibility of a plasmid-induced innate immune response (see Muerdter et al., 2017). Gateway-cassette was added between the KpnI and BglII sites and SCP1 between BglII and HindIII
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Source: Addgene
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