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URL: http://www.addgene.org/64238

Proper Citation: RRID:Addgene_64238

Bacterial Resistance: Ampicillin

Defining Citation: PMID:25721206

Vector Backbone Description: Backbone Marker:Didier Trono; Backbone Size:11101; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin

Comments: The transgene of interest can be inserted into BamHI, PmeI, and PstI sites. However, only the PmeI and PstI sites are recommended to be used for different restriction end ligation. This plasmid must be used together with an independent tTA-expressing plasmid for Tet-Off system or rtTA-expressing plasmid for Tet-On system. The TetO promoter sequence was excised from pETE-Bsd plasmid vector, which was originated from pUHD-10-3. The pETE-Bsd and pUHD-10-13 were engineered by Protopopov et al. and Damke et al, respectively. Protopopov AI, Li J, Winberg G, Gizatullin RZ, Kashuba VI, Klein G, et al. Human cell lines engineered for tetracycline-regulated expression of tumor suppressor candidate genes from a frequently affected chromosomal region, 3p21. J Gene Med. 2002/07/19 ed. 2002;4(4):397–406. Damke, H., Gossen, M., Freundlieb, S., Bujard, H., and Schmid, S.L. (1995). Tightly regulated and inducible expression of dominant interfering dynamin mutant in stably transformed HeLa cells. Methods Enzymol. 257, 209–220.

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