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URL: http://www.addgene.org/64221
Proper Citation: RRID:Addgene_64221
Insert Name: Cas9
Organism: Streptococcus pyogenes
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25803306
Vector Backbone Description: Backbone Marker:Kuhn lab (Addgene plasmid # 64324); Vector Backbone:pU6-(BbsI)_CBh-Cas9-T2A-mCherry; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: Please note that due to the presence of BbsI sites in the Ad4 coding regions it is NOT possible to directly clone new sgRNA sequences into the BbsI sites downstream of the U6 promoter. Therefore, precloned U6-sgRNA cassettes, isolated as 1.7 kb PvuI-XbaI fragments from pU6-(BbsI)_CBh-Cas9-T2A-BFP (plasmid# 64323) or pU6-(BbsI)_CBh-Cas9-T2A-mCherry (plasmid# 64324) of this deposit, or any other plasmid derived from pX330 (plasmid# 42230), must be ligated into the PvuI-XbaI digested backbone of this Ad4 containing plasmid.
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No rating or validation information has been found for pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B.
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Source: Addgene
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