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URL: http://www.addgene.org/60937
Proper Citation: RRID:Addgene_60937
Insert Name: PIP2 dimerization dependent sensor RA+B monomers
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:25622108
Vector Backbone Description: Vector Backbone:pUB2.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Contains a dimerization dependent fluorescent protein, originally described by Alford, S.C., Ding, Y., Simmen, T., and Campbell, R.E. (2012). Dimerization-Dependent Green and Yellow Fluorescent Proteins. ACS Synth. Biol. 1, 569–575. Addgene NGS results identified a frameshift mutation in the HygR gene originally indicated in this plasmid. The depositing lab has confirmed that their experiments were done with transient transfection and did not make use of Hygromycin selection. For users who need a mammalian selection marker, they recommend moving the biosensor coding region from this plasmid into a different plasmid backbone.
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Source: Addgene
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