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URL: http://www.addgene.org/59016
Proper Citation: RRID:Addgene_59016
Insert Name: NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23992616
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Genetically encoded, ratiometric, fluorescent biosensor. Contains nuclear exclusion signal sequence at N-terminus. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins. The Zn(II) binding domain of ZapCY2 is derived from ZapCY1. The first cysteine in each zinc finger is mutated to histidine.
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Source: Addgene
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