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URL: http://www.addgene.org/59011
Proper Citation: RRID:Addgene_59011
Insert Name: NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23173058
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Genetically encoded, ratiometric, fluorescent biosensor. Contains NLS sequence at N-terminus. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains Clover and mRuby fluorescent proteins. The Zn(II) binding domain of ZapCmR1.1 is derived from ZapCY1. The first cysteine in the first zinc finger is mutated to histidine.
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Source: Addgene
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