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URL: http://www.addgene.org/58973
Proper Citation: RRID:Addgene_58973
Insert Name: Oct4
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23910086
Vector Backbone Description: Backbone Marker:Dowdy lab; Vector Backbone:SP6-VEE-IRES-puro (Addgene plasmid 58971); Vector Types:Mammalian Expression, Venezuelan equine encephalitis (VEE) virus RNA replicon; Bacterial Resistance:Ampicillin
Comments: Summary of features: 1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter, nsP1~4 are nonstructural VEE sequences which encode RNA replicases) 7592-8671: human Oct4 8678-8731: T2A peptide sequence 8738-10147: human Klf4 10154-10213: E2A peptide sequence 10223-11176: human Sox2 11195-11805: IRES sequence 11818-13140: human cMyc 13165-13776: IRES sequence 13777-14376: Puromycin resistance gene 14383-14510:VEE 3’UTR and poly A tail 14679-15539: Ampicillin resistance gene 16320-16337: SP6 promoter
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Source: Addgene
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