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Plasmid Name
FW102 OL2-62
RRID:Addgene_53735 RRID Copied  
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RRID:Addgene_53735
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Plasmid Information

URL: http://www.addgene.org/53735

Proper Citation: RRID:Addgene_53735

Insert Name: none

Organism: E.coli

Bacterial Resistance: Kanamycin and Streptomycin

Defining Citation: PMID:9121589

Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Kanamycin and Streptomycin

Comments: FW102 containing an F' Kan bearing the placOL2–62-lacZ fusion where the lambda-CI operator (OL2) is centered at position –62 relative to the start of transcription. This is the reporter strain used with the Hochschild lab bacterial 2-hybrid system, as described in the following article: http://www.addgene.org/browse/article/8393/ This strain is also described in Deighan, P., Diez, C.M., Leibman, M., Hochschild, A., and Nickels, B.E. (2008) The bacteriophage λ Q antiterminator protein contacts the β-flap domain of RNA polymerase, PNAS 105: 15305-15310

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Data and Source Information

Source: Addgene