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URL: http://www.addgene.org/53733
Proper Citation: RRID:Addgene_53733
Insert Name: λCI-β-flap fusion
Organism: Other
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:9121589
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:3142; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Note-When cloning into this plasmid, the 5' end of the insert must provide an extra nucleotide to maintain the reading frame. Additionally, a stop codon must be inserted 5' to the BamHI site. Please see supplemental document for more details. This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids: http://www.addgene.org/browse/article/8393/ The following articles can provide more information on using the bacterial 2-hybrid system: Dove, et al. 1997 PMID 9121589 Dove and Hochschild 1998 PMID 9499408 Deaconescu, et al. 2006 PMID 16469698 Deighan, et al. 2008 PMID 18832144 Kuznedelov, et al. 2002 PMID 11823642
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Source: Addgene
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