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URL: http://www.addgene.org/53284
Proper Citation: RRID:Addgene_53284
Insert Name: pds
Organism: Synechococcus PCC7942
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:17634749
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:13581; Vector Backbone:pAC-EHER; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
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Source: Addgene
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