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URL: http://www.addgene.org/53261
Proper Citation: RRID:Addgene_53261
Insert Name: Cas9-Cys
Organism: S. pyogenes
Bacterial Resistance: Kanamycin
Defining Citation: PMID:24696462
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: A sequence encoding Cas9 with a cysteine at the C-terminus was prepared by PCR amplification using a previously described Cas9 plasmid (Cho et al, Nat Biotechnol. 2013)(Addgene: 43945) as the template and then cloned into the pET28-(a) vector (Novagen, Merk Millipore, Germany), which includes a sequence encoding a His-tag at the N-terminus.
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Source: Addgene
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