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URL: http://www.addgene.org/52676
Proper Citation: RRID:Addgene_52676
Insert Name: ACTN1
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19028690
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Comments: EGFP is a transfection marker, not a fusion. RNAi targeted at 476–494 from the start sequence of rat α-actinin-1 (the sense chain was 5’-gatctccaacgtcaacaagttcaagagacttgttgacgttggagatctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
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Source: Addgene
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