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URL: http://www.addgene.org/52217
Proper Citation: RRID:Addgene_52217
Insert Name: PRHIII-sfGFP
Organism: Synthetic
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:24788326
Vector Backbone Description: Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Plasmid is designed for use in Geobacillus stearothermophilus NUB3621 (as well as E. coli.) Construct is described in the article linked at the bottom of this page. This plasmid is a companion to pIM1708-PsurP-surT-agaN-pNW33N which was previously submitted. As with pIM1708, despite the fact that your plasmid map displays an ampicillin resistance gene, the correct antibiotic resistance gene is a chloramphenicol acetyltransferase. We do not know if the ampicillin resistance gene is functional.
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Source: Addgene
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