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URL: http://www.addgene.org/51290
Proper Citation: RRID:Addgene_51290
Insert Name: L1 ORF1
Organism: Homo sapiens
Bacterial Resistance: Bleocin (Zeocin)
Defining Citation: PMID:22918960
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4595; Vector Backbone:pBudCE4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: Although a myc tag is present in the full sequence, this tag is not present in this plasmid. pBudORF1-CH was generated by cloning the polymerase chain reaction (PCR)-amplified codon-optimized consensus sequences of each ORF into the HindIII-BamHI sites of the pBudCE4.1 vector in a manner that removes the stop codon of the ORF1, so the expressed protein will contain the his tag at the carboxy terminus. The following primers were used in the amplification of ORF1: 5-AGACCCAAGCTTAGCTAAAACCACAAAGATG-3′ and 5-TGTTCGGATCCGATCTTGGTGTGCTTCTGCAGGGG-3′
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Source: Addgene
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