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URL: http://www.addgene.org/47858
Proper Citation: RRID:Addgene_47858
Insert Name: M13_central_T_linker_rrnD_T1 double terminator
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:23474465
Vector Backbone Description: Backbone Size:3967; Vector Backbone:pFAB775; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: Terminator efficiency measured at 100% (97% and 99% alone, respectively). Terminator inserted between RFP and GFP and flanked by Rnase III sites. RNase sites have sequences GTGATAGACTCAAGGTCGCTCCTAGCGAGTGGCCTTTATGATTATCAC and AGAGGGACAAACTCAAGGTCATTCGCAAGAGTGGCCTTTATGATTGACCTTCT, respectively. Terminator can be amplified by PCR with or without these sites, as desired.
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Source: Addgene
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