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URL: http://www.addgene.org/44027
Proper Citation: RRID:Addgene_44027
Insert Name: mHIF-2α (P405A/P530V/N851A)
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17804822
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid encodes an oxygen-regulation insensitive (normoxia-stable and active) full-length murine HIF-2α. Full-length murine HIF-2α cDNA was generated by reverse transcription-PCR and inserted into a pcDNA3 expression vector by using primers incorporated with restriction sites. A 10-amino-acid c-myc tag was further inserted at the C terminus of the HIF-2α cDNAs by using a Pfu-PCR-based protocol. The same Pfu-PCR protocol was then used to change residues Proline 405, Proline 530 and Asparagine 851. The newly constructed plasmid was sequenced to confirm encoding of the correct protein. This plasmid has an A94V polymorphism that does not affect protein function. Alternate plasmid names: HIF-2α triple mutant (TM); pcDNA3 HIF-2α TM
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No rating or validation information has been found for pcDNA3 mHIF-2α MYC (P405A/P530V/N851A).
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Source: Addgene
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