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URL: http://www.addgene.org/43975
Proper Citation: RRID:Addgene_43975
Bacterial Resistance: Kanamycin
Defining Citation: PMID:23410102
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5749; Vector Backbone:pET, pCoofy1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. Note that depending on the protein, the 10x His tag can reduce or abolish expression.
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Source: Addgene
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