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URL: http://www.addgene.org/40237
Proper Citation: RRID:Addgene_40237
Insert Name: promoterless luxAB reporter
Organism: Synechococcus elongatus PCC 7942
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:10812624
Vector Backbone Description: Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin
Comments: NS1 vector Lux Genes: luxAB cloning sites NotI and BamHI Blunted (by Klenow) SalI-PvuII luxAB fragment (from pLAV1) was cloned in the SmaI site of pAM1303. SalI site is approximately 135bp upstream of luxA ATG within the luxD reading frame. You can clone promoters upstream of luxAB in NotI or BamHI sites.
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Source: Addgene