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URL: http://www.addgene.org/38253
Proper Citation: RRID:Addgene_38253
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19956581
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:6153; Vector Backbone:pET22b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: For protein purification, target protein of interest should be cloned into NdeI and SalI sites to create HA-tagged fusion with Vibrio cholerae MARTX toxin cysteine protease domain (CPD). HA epitope tag will remain on target protein after cleavage.
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Source: Addgene
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