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URL: http://www.addgene.org/38209
Proper Citation: RRID:Addgene_38209
Insert Name: Bxb1 Integrase
Bacterial Resistance: Kanamycin
Defining Citation: PMID:22615351
Vector Backbone Description: Vector Backbone:pSB3K1; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Kanamycin
Comments: The Bxb1 integrase was cloned downstream of the PBAD/AraC promoter (BBa_I0500) on pSB3K1 plasmid bearing a p15A origin of replication (15-20 copies). This version of the integrase has a 6-His-tag which we found to stabilize the protein. Therefore, a weak RBS (BBa_B0031) and a LAA ssrA tag was added to reduce the basal expression of the enzyme.
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Source: Addgene
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