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URL: http://www.addgene.org/38190
Proper Citation: RRID:Addgene_38190
Insert Name: ABR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:4805; Vector Backbone:pKH3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Young Jin Cho. A human ABR cDNA was inserted into pKH3 (Addgene plasmid 12555; obtained from Martin Schwartz) digested with BamHI x EcoRI in a three-way ligation (0.4 kb BamHI-BstEII + 2.2 kb BstEII-EcoRI). The 5’ sequence is GGA TCC ATG-start ABR. Note: This plasmid may contain an E24G mutation -gag to ggg- introduced by Taq polymerase at an earlier cloning step.
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Source: Addgene