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URL: http://www.addgene.org/38182
Proper Citation: RRID:Addgene_38182
Insert Name: ABR DH domain N282AE283A
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17116687
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGEX 3X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Young Jin Cho. Mutagenesis done using Stratagene Quick Change kit. 0.35 kb Bgl IIxSstI fragment containing the N282A/E283A double mutation from Abr EYFP (AddGene #36419) was ligated into the "ABR DH domain pGEX" plasmid (AddGene #38181) digested with Bgl II x SstI and removal of the 0.35 kb fragment containing the wt sequence N282E283. The amino acid numbering is as in NM_021962 (isoform A).
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Source: Addgene
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