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URL: http://www.addgene.org/38176
Proper Citation: RRID:Addgene_38176
Insert Name: BCR GAP domain encoding residues 1004-1271 with R1090A point mutation
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17116687
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Young Jin Cho. pcDNA3.1Xpress-BcrGAP plasmid(AddGene #38175) was digested by BstEII x EcoRI and a 1.2 kb fragment containing the R1090 wt site, TAG and 3’ UT sequences removed. The wt fragment was replaced by a 0.6 kb BstEII x EcoRI fragment containing the R1090A mutation isolated from EGFP-BCR R1090A pEGFP (AddGene #38173). The construct contains the natural TAG and 3’ UT sequences. The difference between wt and mutant plasmids is in the 3’ UT region. The construct includes amino acid residues 1004-1271.
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Source: Addgene
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