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URL: http://www.addgene.org/38157
Proper Citation: RRID:Addgene_38157
Insert Name: ABR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18070886
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pLEF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.4 kb BamHI/BstEII + 3' 2.2 kb BstEII/EcoRI ABR fragments into pLEF digested with BamHIxEcoRI. The ABR insert contains the natural TAG and 3' UT sequences. Addgene sequencing found an E24G point mutation--this does not alter plasmid function.
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Source: Addgene