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URL: http://www.addgene.org/37864
Proper Citation: RRID:Addgene_37864
Insert Name: BKV ER
Organism: BK polyomavirus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22810586
Vector Backbone Description: Backbone Size:8162; Vector Backbone:MSCV-N FLAGHA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: There is a splice site in the DNA to make a truncated Lg T (348bp), as well as a full length small T, both tagged at N-terminal end. The small and Lg T seqences overlap for the first 242 bp. (genome entry, BKV, strain UT: https://www.ncbi.nlm.nih.gov/nucleotide/83415612?report=genbank&log$=nuclalign&blast_rank=4&RID=53X3T5WA016 ) Both proteins are seen when this construct is expressed in cells.
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Source: Addgene
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