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Plasmid Name
pBAD-D2
RRID:Addgene_37470 RRID Copied  
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RRID:Addgene_37470
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Plasmid Information

URL: http://www.addgene.org/37470

Proper Citation: RRID:Addgene_37470

Insert Name: D2 second generation cameleon (calcium sensor)

Organism: Synthetic

Bacterial Resistance: Ampicillin

Defining Citation: PMID:16720273

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin

Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and citrine. D2 does not bind wild-type calmodulin. It has a Kd1' and Kd2' of 0.8 microM and 28 microM. See also Palmer & Tsien (2006) Nature Protocols.

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Data and Source Information

Source: Addgene