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Plasmid Name
pDZ306 (GLT1 POL1 24xPP7SL)
RRID:Addgene_35196 RRID Copied  
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RRID:Addgene_35196
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Plasmid Information

URL: http://www.addgene.org/35196

Proper Citation: RRID:Addgene_35196

Insert Name: GLT1 POL1 24xPP7SL

Organism: Synthetic

Bacterial Resistance: Ampicillin and Kanamycin

Defining Citation: PMID:21512033

Vector Backbone Description: Vector Backbone:pHIS; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin

Comments: Note that Addgene NGS results found 22xPP7 stem loop repeats rather than expected 24. It is not known how this impacts plasmid function. This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid. Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.

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Data and Source Information

Source: Addgene