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URL: http://www.addgene.org/33372
Proper Citation: RRID:Addgene_33372
Insert Name: MBP based Maltose Biosensor
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21989929
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The MBP-cpGFP insertion variant was constructed by overlap PCR using the wild-type MBP sequence from the pMalE-p4E vector (New England Biolabs) and the cpGFP146 variant from GCaMP2 into pRSET-A between BamHI and EcoRI sites. The N-terminal periplasmic leader sequence of MBP was excluded, and a BamHI site (encoding Gly-Ser) was included at the 5′-end. At the C-terminus, an additional His6Gly tag was included after the terminus of MBP (a cloning oversight). An EcoRI site was included after the stop codon.
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Source: Addgene
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