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URL: http://www.addgene.org/33000
Proper Citation: RRID:Addgene_33000
Insert Name: H2B-PAGFP
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:22144948
Vector Backbone Description: Backbone Size:4771; Vector Backbone:N/A; Vector Types:Mammalian Expression, AAV, adeno associated virus; Bacterial Resistance:Ampicillin
Comments: Plasmid containing the H2B-PAGFP fusion gene was a gift from André Nussenzweig (Kruhlak et al., 2006). The H2B-PAGFP gene was subcloned into the pACAGW-ChR2-Venus-AAV plasmid (Addgene # 20071). Kruhlak, M. J., Celeste, A., Dellaire, G., Fernandez-Capetillo, O., Müller, W. G., McNally, J. G., Bazett-Jones, D. P., and Nussenzweig, A. (2006). Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks. J. Cell Biol. 172, 823-34. There is a 5bp deletion in Addgene's sequence compared to depositor's reference sequence. The deletion is in the vector sequence upstream of the ORF and does not affect plasmid function. There is also a mutation D26G in H2B; this mutation is found in depositor's reference sequence as well.
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Source: Addgene
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